Journal
BIOPHYSICAL JOURNAL
Volume 116, Issue 2, Pages 239-247Publisher
CELL PRESS
DOI: 10.1016/j.bpj.2018.11.3132
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Funding
- Fondazione Cassa di Risparmio di Firenze, Ministero dell'Istruzione, dell'Universita e della Ricerca (MIUR) PRIN [2012SK7ASN]
- MEDINTECH [CTN01_001177_962865]
- EC [653706, 675858, 731005]
- PhosAgro/UNESCO/IUPAC Green Chemistry for Life [4500319942]
- University of Florence CERM-TT, Recombinant Proteins JOYNLAB
- Instruct-ERIC
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FIowNMR has the aim of continuously monitoring processes that occur in conditions that are not compatible with being carried out within a closed tube. However, it is sample intensive and not suitable for samples, such as proteins or living cells, that are often available in limited volumes and possibly low concentrations. We here propose a dialysis-based modification of a commercial flowNMR setup that allows for recycling the medium while confining the sample (proteins and cells) within the active volume of the tube. This approach is demonstrated in the specific cases of in-cell NMR and protein-based ligand studies.
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