Journal
BIOCONJUGATE CHEMISTRY
Volume 29, Issue 12, Pages 4030-4039Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.8b00599
Keywords
-
Categories
Funding
- National Institute of General Medical Science [GM62444]
Ask authors/readers for more resources
Chemoenzymatic synthesis is an important strategy for the formation of glycopolymers. The use of a smaller number of traditional chemical steps and enzyme catalyzed reactions increases the yield of glycopolymer that can be produced by reducing the overall number of synthetic steps. In addition, chemoenzymatic routes are likely to be more accessible to those without a background in carbohydrate synthesis, making glycopolymers more available for studies across a broader range of scientists. Here, the enzymatic addition of galactose to N-acetylglucosamine functionalized glycodendrimers reduced the requisite number of synthetic steps for the full chemical synthesis of N-acetyl lactosamine (LacNAc) functionalized dendrimers to four steps. Unpurified cell lysate was used in the enzyme catalyzed glycosylation, and product glycodendrimers were readily purified by dialysis after enzymatic degradation of all protein components of the lysate in the crude reaction mixture. LacNAc functionalized dendrimers were used very effectively in homotypic cancer cellular aggregation assays and were found to either inhibit or enhance galectin-3 mediated cancer cellular aggregation, with differences in outcomes dependent on the generation of LacNAc functionalized dendrimers that were used.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available