Journal
BIOCONJUGATE CHEMISTRY
Volume 29, Issue 11, Pages 3847-3855Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.8b00678
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Funding
- NIH Common Fund Glyco-science [U01GM116254]
- STTR grant [R41GM122139]
- Emory University School of Medicine
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The advancement of glycoscience is critically dependent on the access to a large number of glycans for their functional study. Naturally occurring glycans are considered a viable source for diverse and biologically relevant glycan libraries. A mixture of free reducing glycans released from natural sources can be fluorescently tagged and separated by chromatography to produce a natural glycan library. Anthranilic acid (AA) has been widely used to fluorescently tag reducing glycans for HPLC or LC/MS analysis. However, AA conjugated glycans are not efficiently immobilized on microarray slides due to the lack of a primary alkylamine functional group. In this study, we have developed simple and efficient chemistry for bioconjugation and further functionalization of glycan-AA conjugates. This new approach enables quick preparation of glycan microarrays and neoglycoproteins from glycan-AA conjugates, which can be separated by weak anion exchange (WAX) and C18 reversed-phase HPLC.
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