Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
Volume 1862, Issue 1, Pages 35-46Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbagrm.2018.10.008
Keywords
ALKBH3; m(1)A demethylation; CSF-1 mRNA; RNA stability; Ovarian cancer; Breast cancer
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Funding
- Women's Cancers of the University of Arizona Cancer Center
- Bobbi Olson Endowment Fund
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In ovarian and breast cancers, the actions of the cytokine CSF-1 lead to poor prognosis. CSF-1 expression can be regulated post-transcriptionally. RNA methylation is another layer of posttranscriptional regulation. The methylation of N-1 atom of adenine (m(1)A) results in a conformational change of RNA which regulates translational efficiency. Our study indicates that the m(1)A is also involved in the CSF-1 mRNA decay. The alteration of ALKBH3 expression, an m(1)A demethylase, regulates the CSF-1 mRNA stability. Demethylation of m(1)A by ALKBH3 increases the half-life of CSF-1 mRNA without affecting the translation efficiency. The m(1)A in CSF-1 mRNA is mapped in the 5'UTR near the translation initiation site. YTHDF2, a known m(6)A reader which interacts with the CCR4-NOT deadenylation complex, is not the reader of m(1)A-containing CSF-1 mRNA. Overexpression of ALKBH3 increases CSF-1 expression and the degree of cancer cell invasiveness without affecting cell proliferation or migration. Collectively, we showed that CSF-1 mRNA decay can be regulated at an epigenetic level, and that alteration of the N-1-methylation status leads to phenotypic changes in cancer cell behavior.
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