Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 506, Issue 3, Pages 703-708Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.10.123
Keywords
SRSF4; Pre-mRNA splicing; Alternative splicing; Fas; Exon 6
Categories
Funding
- National Research Foundation of Korea
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute - Ministry of Health & Welfare, Republic of Korea [HI17C0196]
- Cell Logistics Research Center - Ministry of Education of the Republic of Korea [2016R1A5A1007318]
- [NRF-2017R1A2B2005896]
- [NRF-2016R1A2B1007135]
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Alternative splicing of exon 6 in Fas pre-mRNA generates a membrane bound pro-apoptotic isoform or soluble anti-apoptotic isoform. SRSF4 is a member of Arginine-Serine rich (SR) protein family. Here we demonstrate that increased SRSF4 expression stimulates exon 6 inclusion, and that reduced SRSF4 expression promotes exon 6 exclusion. We also show that weaker but not stronger 5' splice-site strength of exon 6 abolishes the SRSF4 effects on exon 6 splicing. Furthermore, we identified a novel enhancer on exon 6, on which SRSF4 interacts functionally and physically. Our results illustrate a novel regulatory mechanism of Fas pre-mRNA splicing. (C) 2018 Elsevier Inc. All rights reserved.
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