Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 508, Issue 1, Pages 250-255Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2018.11.087
Keywords
PETase; Ideonella sakaiensis; Membrane translocation; Extracellular protein production; E. coli; Signal peptide
Categories
Funding
- Next Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ01326503]
- Ministry of Science, ICT and Future Planning through the National Research Foundation of Korea [NRF-2017M1A2A2087631]
- Global PhD Fellowship Program of the Korean Government [2018H1A2A1061751, 2015H1A2A1034233, 2017H1A2A1042052]
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Poly(ethylene terephthalate) (PET) is the most commonly used polyester polymer resin in fabrics and storage materials, and its accumulation in the environment is a global problem. The ability of PET hydrolase from Ideonella sakaiensis 201-F6 (IsPETase) to degrade PET at moderate temperatures has been studied extensively. However, due to its low structural stability and solubility, it is difficult to apply standard laboratory-level IsPETase expression and purification procedures in industry. To overcome this difficulty, the expression of IsPETase can be improved by using a secretion system. This is the first report on the production of an extracellular IsPETase, active against PET film, using Sec-dependent translocation signal peptides from E. coli. In this work, we tested the effects of fusions of the Sec-dependent and SRP dependent signal peptides from E. coli secretory proteins into IsPETase, and successfully produced the extracellular enzyme using pET22b-SPMalE:IsPETase and pET22b-SP (LamB) :IsPETase expression systems. We also confirmed that the secreted IsPETase has PET-degradation activity. The work will be used for development of a new E. coli strain capable of degrading and assimilating PET in its culture medium. (C) 2018 Elsevier Inc. All rights reserved.
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