Journal
MITOCHONDRIAL DNA PART A
Volume 27, Issue 6, Pages 4112-4113Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/19401736.2014.1003868
Keywords
GenBank; illumina sequencing; MITObim; mitogenome; silk moth
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Funding
- Natural Science Basic Research Plan in Shaanxi Province of China [2013JQ3007]
- Fundamental Research Funds for the Central Universities in China [GK201302020, GK201402030]
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The complete mitochondrial genome of the wild silk moth Bombyx huttoni has been reconstructed from the whole-genome Illumina sequencing data. The circular genome was determined to be 15,638 bp in length, consisting of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one A+T-rich D-loop or control region. Twelve PCGs (COX2, COX3, CYTB, ND1, ND2, ND3, ND4, ND4L, ND5, ND6, ATP6, and ATP) are initiated with the typical ATN codons, whereas COX1 was tentatively determined to use the unusual CGA as its start codon. All PCGs are terminated with the TAA codon, except for ND1 and the other two PCGs (COX1 and COX2) with the TAG and the incomplete T stop codon, respectively. The nucleotide composition is highly asymmetric (43.8% A, 38.0% T, 7.1% G, and 11.1% C) with an overall A+T content of 81.8%.
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