Journal
ARCHIVES OF VIROLOGY
Volume 164, Issue 3, Pages 907-911Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-018-04133-5
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Funding
- National Natural Science Foundation of China [81273138, 81572045]
- Yunnan Health Training Project of High-Level Talents [D-201604]
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A novel negevirus, tentatively named Manglie virus (MaV), was isolated from Culex tritaeniorhynchus from the village of Manglie, Yunnan, China, in August 2011. It was identified by high-throughput sequencing of cell culture supernatants, and the complete genome was sequenced using an Illumina MiSeq sequencer. The complete MaV genome comprised 9,218 nt encoding three hypothetical proteins and had a poly(A) tail. BLASTn analysis showed that the genome had the greatest similarity to Ngewotan virus strain Nepal22, with query coverage of 100% and 79% identity. Genomic and phylogenetic analyses demonstrated that MaV should be considered a novel negevirus.
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