Journal
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
Volume 100, Issue 1, Pages -Publisher
WILEY
DOI: 10.1002/arch.21511
Keywords
apoptosis; lncRNAs; miRNAs; silkgland; silkworm Bombyx mori (B. mori)
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Funding
- National Natural Science Foundation of China [31372374/C1703, 31672491/C1703]
- National Basic Research Program of China [2012CB114601]
- Science and Technology Innovation Team of Zhejiang Province [2010R50031]
- Chinese Universities Scientific Fund
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The Silkworm Bombyx mori is an important insect in terms of economics and a model organism with a complete metamorphosis. The economic importance of silkworms is dependent on the functions of the silkgland, a specialized organ that synthesizes silk proteins. The silk gland undergoes massive degeneration during the larval to pupal stage, which involves in cell apoptosis. In this paper, high throughput sequencing was used to detect the expression of messenger RNA (mRNA), long noncoding RNA (lncRNA), and microRNA (miRNA) from silk glands of Day 3 in the fifth instar larvae (L5D3) and the spinning 36h (sp36h). We analyzed the Gene Ontology (GO) functions of target genes of the differentially expressed lncRNAs and miRNAs. We investigated the regulations of mRNA, lncRNA, and miRNA on silk gland apoptosis in L5D3 and sp36h. In total, 10,947 lncRNAs were detected in the silk gland and the index number TCONS-00021360 lncRNA may be involved in the process of apoptosis. In addition, 344 miRNAs targeted 285 mRNAs were related to the death process under GO entry. The results indicated that miRNAs play an important role in the molecular regulation of the silk gland apoptosis compared with that of lncRNAs. Finally, we screened 746 lncRNAs and 20 miRNAs that might interact with BmDredd, and drew an interaction network among them.
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