Dichlorprop induced structural changes of LHCII chiral macroaggregates associated with enantioselective toxicity to Scnedesmus obliquus

The enantioselective toxic mechanisms of chiral herbicides in photosynthetic organisms are closely related to the production of reactive oxygen species (ROS) production, however, there are few reports on how the enantioselective production of ROS can be triggered. In suboptimal conditions, photosynthesis is one of the most important processes in the production of ROS, especially in the process of light utilization and electron transfer. In this study, we investigated the interactions between chiral herbicide dichlorprop (DCPP) enantiomers and the chiral macroaggregates of the photosynthetic light-harvesting chlorophyll a/b pigment-protein complexes (LHCII) in Scenedesmus obliquus, which is of great significance in capturing and utilizing sun light, and also in dissipating the excess excitation energy. The results of the circular dichroism indicated that DCPP induced the structural changes of the LHCII chiral macroaggregates in an enantioselective manner and that the (R)-DCPP treated-group showed a bigger change accompanied by a changed enantioselective dissipation of the excitation energy. The excitation energy was excessed in DCPP treated-groups and the degree of excess was enantioselective and the detrimental non-chemical energy triggered the enantioselective production of ROS, that induced the enantioselective toxicity to green algae S. obliquus. Overall, this study has identified that how the enantioselective production of ROS can be triggered in chloroplasts; this can help to reveal the enantioselective mechanisms of chiral herbicides to photosynthetic organisms.