Effects of autochthonous probiotics, isolated from Tor grypus (Karaman, 1971) intestine and Lactobacillus casei (PTCC 1608) on expression of immune-related genes

In this study, Lactobacillus plantarum subsp. plantarum and L. delbrukei subsp. bulgaricus were used as autochthonous food supplementation for gene expression and hematological investigation. Then, 480 fish (BW, 45 +/- 10 g) were randomly divided into four groups (three replications) and were fed with diet containing 5 x 10(7) CFU g(-1) of each isolated LAB and L. casei (PTCC 1608) and a control diet with no bacteria. Feeding trail was conducted for 60 days, following a change to the non-supplemented feed until 15 days (day 75). Samples from blood and head kidney at days 0, 60, and 75 were taken. The in vitro examination showed that L. plantarum had high antimicrobial activity against the Aeromonas hydrophila and Yesinia ruckeri. Results of this study showed that feeding of T. grypus with L. plantarum supplementation diet led to increase Hb and WBC, and induce TNF-alpha, IL-8, and IL-1 beta expressions in the head kidney rather than control. Results suggested that L. plantarum can be applied as a dietary supplement to enhance the immunity system of T. grypus.