Journal
ANNUAL REVIEW OF BIOCHEMISTRY, VOL 88
Volume 88, Issue -, Pages 635-659Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev-biochem-013118-110801
Keywords
single-molecule microscopy; single-molecule kinetics; fluorescence labeling; single-molecule tracking; spot localization; simulated microscopy
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In the past decades, advances in microscopy have made it possible to study the dynamics of individual biomolecules in vitro and resolve intramolecular kinetics that would otherwise be hidden in ensemble averages. More recently, single-molecule methods have been used to image, localize, and track individually labeled macromolecules in the cytoplasm of living cells, allowing investigations of intermolecular kinetics under physiologically relevant conditions. In this review, we illuminate the particular advantages of single-molecule techniques when studying kinetics in living cells and discuss solutions to specific challenges associated with these methods.
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