Journal
ANIMAL SCIENCE JOURNAL
Volume 90, Issue 1, Pages 117-127Publisher
WILEY
DOI: 10.1111/asj.13133
Keywords
Astragalus polysaccharides; broiler; immune function; irradiation; physicochemical property
Categories
Funding
- National Natural Science Foundation of China [31601957]
- Fundamental Research Funds for the Central Universities of China [KJQN201723]
- National Key Research and Development Program of China [2017YFD0500505]
- Earmarked Fund for Jiangsu Agricultural Industry Technology System [JATS2018282]
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In this study, we irradiated Astragalus polysaccharides (APS) using 25 kGy Co-60 gamma ray to obtain gamma-irradiated Astragalus polysaccharides (IAPS) and then investigated the effects of IAPS on growth performance and immune function of cyclophosphamide (CPM)-treated broilers. The physicochemical properties of APS and IAPS (molecular weight, water solubility, viscosity, morphological and structural properties) were evaluated. Then, 384 one-day-old Arbor Acres broiler chicks with similar initial weight were randomly assigned into 6 groups: the non-treated group (control), and CPM-treated groups were fed either a basal diet or the diets containing 900 mg/kg APS, or 900, 600, 300 mg/kg IAPS, respectively. On days 16, 18, and 20, all broilers except for the control group were intramuscularly injected with 0.5 ml CPM (40 mg/kg center dot BW). Broilers in the control group were intramuscularly injected with 0.5 ml sterilized saline (0.75%, wt/vol). This trial lasted for 21 days. The physicochemical treatment showed that gamma irradiation could decrease the molecular weight and viscosity, and increase the water solubility of APS (p < 0.05), whereas the structural properties of APS was not affected. In the animal trial, 900 mg/kg APS or 900, 600 mg/kg IAPS relieved the decreased growth performance, thymus index, T lymphocytes proliferation, serum IgG concentration, NOS activity and the increased blood heterophil:lymphocyte ratio in CPM-treated broilers (p < 0.05). CPM-induced decreases in B lymphocytes proliferation and serum IgM concentration were only increased by IAPS at 900 mg/kg (p < 0.05). Overall, both APS and IAPS alleviated CPM-induced immunosuppression. Especially, IAPS possessed better immunomodulatory effect than APS, indicating that gamma irradiation could be used as an effective method to enhance the immunomodulatory activity of APS.
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