Journal
ANDROLOGIA
Volume 51, Issue 4, Pages -Publisher
WILEY
DOI: 10.1111/and.13222
Keywords
biomarker; proteomics; seminal plasma; sperm morphology; sperm motility; swine
Categories
Funding
- FUVATES
- Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul [16/2551-3360000223-1]
- Fundacao Cearense de Apoio ao Desenvolvimento Cientifico e Tecnologico
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [447251/2014-7]
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The identification of biomarkers associated with seminal traits could aid in the selection of higher quality ejaculates and benefit the swine industry. The objective of this study was to identify boar seminal plasma proteins associated with sperm motility and morphology. Twenty ejaculates from fifteen adult boars from a commercial boar stud were used for this work. After routine semen collection and analysis, ejaculates were classified into two groups: high-quality semen (HQS) and low-quality semen (LQS), based on sperm motility and morphology. Semen samples were processed for seminal plasma separation and analysis by 2D SDS-PAGE. Total and progressive sperm motility differed between groups (p < 0.001), as well sperm morphology (p < 0.05). The intensity of spots identified as Major seminal plasma PSP-I (PSP-I) and cathepsin B (CTSB) was higher in LQS as compared to HQS samples (p < 0.05). Also, PSP-I was positively associated with major and sperm cauda defects. Sperm motility was negatively correlated with both PSP-I and cathepsin B. We conclude that high concentrations of Major seminal plasma PSP-I and cathepsin B in boar seminal plasma are associated with reduced total and progressive sperm motility and low sperm morphology and might be used as biomarkers for semen quality.
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