Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 21, Pages 13059-13064Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b04055
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21575031, 21775030, 21765003]
- Natural Science Foundation of Guangxi Province [2015GXNSFDA139006, 2017GXNSFFA198014]
- BAGUI Scholar Program
Ask authors/readers for more resources
Calcium ion (Ca2+) plays crucial roles in the signal transduction pathways associated with various physiological and pathological events. Monitoring intracellular Ca2+ is of great significance for cell biology research. Here, we report the use of biomass quantum dots (BQDs) as a fluorescent reporter for imaging of intracellular Ca2+, based on the inner-filter-mediated luminescence which was assisted by a Ca2+ chelator alizarin red S (ARS). BQDs were prepared by hydrothermal heating of capsicum. The absorption of ARS overlaps with the excitation of the BQDs, the fluorescence of BQDs being quenched through the inner-filter effect. But the absorption of Ca-ARS complex red shifts and shows a poor inner-filter effect. Thus, Ca2+ can be detected by the inner-filter-mediated luminescence using the BQDs-ARS nanohybrid system. Using the proposed nanosystem, the imaging of intracellular Ca2+ and real-time monitoring of the Ca2+ level change under histamine stimulation were also achieved. Thus, this nanosystem holds potential applications in other Ca2+-related signal transduction study.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available