Journal
ANALYTICAL CHEMISTRY
Volume 90, Issue 24, Pages 14402-14411Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.8b03959
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Funding
- National Natural Science Foundation of China [21775034, 21475034, 21675041]
- Science Foundation for Distinguished Young Scholars of Hubei Province [2018CFA041]
- Hubei Province health and family planning scientific research project [WJ2017Q032]
- Hubei Provincial Key Laboratory of Occurrence and Intervention of Rhumatic Diseases [OIR15001]
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Tumor exosomes that inherit molecular markers from their parent cells are emerging as cellular surrogates in cancer diagnostics. Molecular profiling and detection of exosomes offer a noninvasive access to the state of cancer progression, yet are still technically challenging. Here we report an exosome-oriented, aptamer nanoprobe-based profiling (ExoAPP) assay to phenotype surface proteins and quantify cancerous exosomes in a facile mix-and-detect format. Our ExoAPP interfaces graphene oxide (GO) with target-responsive aptamers to profile exosomal markers across five cell types by complementing with enzyme assisted exosome recycling, revealing a heterogeneous pattern. This assay achieves a detection limit down to 1.6 x 10(5) particles/mL, lowered by several orders of magnitude over other homogeneous protocols. Such a sensitive ExoAPP assay allows for monitoring epithelial-mesenchymal transition through heterogeneous exosomes without involving cellular internalization that often occurs in GO-based cargo delivery. Using ExoAPP to analyze blood samples from prostate cancer patients, we find that target exosome can be identified by surface PSMA, suggesting their potential in clinical diagnosis.
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