Journal
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
Volume 316, Issue 1, Pages H224-H232Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.00507.2018
Keywords
alpha(1)-adrenergic; ERK; heart failure; magnetic resonance imaging; myofilament; right ventricle; reactive oxygen species; therapy
Funding
- Department of Veterans Affairs Merit Review Awards [I01BX000740, I01BX001970]
- Department of Veterans Affairs Shared Equipment Grant [1IS1BX003101-01]
- National Heart, Lung, and Blood Institute [HL-31113]
- University of California-San Franciso Resource Allocation Program
- American Heart Association [16POST30970031]
- [15GRNT25550041]
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Right ventricular (RV) failure (RVF) is a serious disease with no effective treatment available. We recently reported a disease prevention study showing that chronic stimulation of alpha(1A)-adrenergic receptors (alpha(1A)-ARs), started at the time of RV injury, prevented the development of RVF. The present study used a clinically relevant disease reversal design to test if chronic alpha(1A)-AR stimulation, started after RVF was established, could reverse RVF. RVF was induced surgically by pulmonary artery constriction in mice. Two weeks after pulmonary artery constriction, in vivo RV fractional shortening as assessed by MRI was reduced by half relative to sham-operated controls (25 +/- 2%, n = 27, vs. 52 +/- 2%, n = 13, P < 10-(11)). Subsequent chronic treatment with the alpha(1A)-AR agonist A61603 for a further 2 wk resulted in a substantial recovery of RV fractional shortening (to 41 +/- 2%, n = 17, P < 10(-7) by a paired t-test) along with recovery of voluntary exercise capacity. Mechanistically, chronic A61603 treatment resulted in increased activation of the prosurvival kinase ERK, increased abundance of the antiapoptosis factor Bcl-2, and decreased myocyte necrosis evidenced by a decreased serum level of cardiac troponin. Moreover, A61603 treatment caused increased abundance of the antioxidant glutathione peroxidase-1, decreased level of reactive oxygen species, and decreased oxidative modification (carbonylation) of myofilament proteins. Consistent with these effects, A61603 treatment resulted in increased force development by cardiac myofilaments, which might have contributed to increased RV function. These findings suggest that the alpha(1A)-AR is a therapeutic target to reverse established RVF. NEW & NOTEWORTHY Currently, there are no effective therapies for right ventricular (RV) failure (RVF). This project evaluated a novel therapy for RVF. In a mouse model of RVF, chronic stimulation of alpha(1A)-adrenergic receptors with the agonist A61603 resulted in recovery of in vivo RV function, improved exercise capacity, reduced oxidative stress-related carbonylation of contractile proteins, and increased myofilament force generation. These results suggest that the alpha(1A)-adrenergic receptor is a therapeutic target to treat RVF.
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