Journal
ADVANCED FUNCTIONAL MATERIALS
Volume 29, Issue 1, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201807168
Keywords
biomineralization; calcite; coccolithophores; cryo-transmission electron microscopy; crystal nucleation
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Funding
- University of Edinburgh
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/M029611/1]
- European Research Council (ERC) Starting Grant [SP2-GA-2008-200915]
- Wellcome Trust [WT087658]
- Scottish Universities Life Sciences Alliance (SULSA)
- BBSRC [BB/M029611/1] Funding Source: UKRI
- NERC [NE/H017119/1] Funding Source: UKRI
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Coccolith-associated polysaccharides (CAPs) are thought to be a key part of the biomineralization process in coccolithophores; however, their role is not fully understood. Two different systems that promote different polymorphs of calcium carbonate are used to show the effect of CAPs on nucleation and polymorph selection in vitro. Using a combination of time-resolved cryo-transmission electron microscopy and scanning electron microscopy, the mechanisms of calcite nucleation and growth in the presence of the intracrystalline fraction are examined containing CAPs extracted from coccoliths from Gephyrocapsa oceanica and Emiliania huxleyi, two closely related coccolithophore species. The CAPs extracted from G. oceanica are shown to promote calcite nucleation in vitro, even under conditions favoring the kinetic products of calcium carbonate, vaterite, and aragonite. This is not the case with CAPs extracted from E. huxleyi, suggesting that the functional role of CAPs in vivo may be different between the two species. Additionally, high-resolution synchrotron powder X-ray diffraction has revealed that the polysaccharide is located between grain boundaries of both calcite produced in the presence of the CAPs in vitro and biogenic calcite, rather than within the crystal lattice.
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