Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 4, Pages 3745-3752Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b20144
Keywords
DNA methylation; hybridization chain reaction; biosensor; DNA tetrahedral nanostructure probe; multiple signal amplification
Funding
- National Natural Science Foundation of China [81572078, 81401722, 81873982]
- Third Military Medical University Medical Creative Research Foundation [SWH2016JCYB-62, SWH2016JCYB-33]
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DNA methylation is a key factor in the pathogenesis of gene expression diseases or malignancies. Thus, it has become a significant biomarker for the diagnosis and prognosis of these diseases. In this paper, we designed an ultrasensitive and specific electrochemical biosensor for DNA methylation detection. The platform consisted of stem-loop-tetrahedron composite DNA probes anchoring at a Au nanoparticle-coated gold electrode, a restriction enzyme digestion of HpaII, and signal amplification procedures including electrodeposition of Au nanoparticles, hybridization chain reaction, and horseradish peroxidase enzymatic catalysis. Under optimal conditions, the design showed a broad dynamic range from 1 aM to 1 pM and a detection limit of about 0.93 aM. The approach also showed ideal specificity, repeatability, and stability. The recovery test demonstrated that the design is a promising platform for DNA methylation detection under clinical circumstances and could meet the need for cancer diagnosis.
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