Journal
3 BIOTECH
Volume 5, Issue 4, Pages 411-421Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-014-0236-7
Keywords
Agro-industrial waste; Halotolerant; Solar saltern; Aspergillus tubingensis; Deproteinized acid cheese whey; Response surface methodology
Categories
Ask authors/readers for more resources
A halotolerant fungal isolate Aspergillus tubingensis GR1 was isolated from the man-made solar saltern located at Khambhat, Gujarat, India, and identified using 28S rDNA partial genome sequencing. This isolate was studied for beta-galactosidase production under solid state fermentation using wheat bran and deproteinized acid cheese whey. The influence of various agro-industrial wastes, nitrogen source and other growth conditions on beta-galactosidase production was investigated using 'one-factor-at-a-time' approach. Among various variables screened along with wheat bran and deproteinized acid cheese whey as major growth substrate, corn steep liquor and MgSO4 were found to be most significant. The optimum concentrations of these significant parameters were determined employing the response surface central composite design, revealing corn steep liquor concentration (2 mL) and magnesium sulphate (50 mg) per 5 g of wheat bran and 20 mL of deproteinized acid cheese whey for highest enzyme production (15,936 U/gds). These results suggest the feasibility of industrial large-scale production of beta-galactosidase known to be valuable in whey hydrolysis and removal of galactosyl residue from polysaccharide.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available