Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 94, Issue -, Pages 1-8Publisher
ELSEVIER
DOI: 10.1016/j.bej.2014.11.004
Keywords
Lactic acid; Ion exchange; Fermentation; Adsorption; Corn stover; Product inhibition
Funding
- United States Department of Agriculture (USDA)-National Institute of Food and Agriculture (NIFA) under the Northwest Advanced Renewables Alliance (NARA) [2011-68005-30416]
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A thermotolerant, mixed culture with Bacillus coagulans as a dominating bacterium was grown for production of lactic acid. Acid extraction using Amberlite (TM) IRA-67 weak base resin in a recirculation loop with a fermentation vessel was implemented to maximize culture productivity by maintaining a concentration of lactic acid below 20 g/L. Productivity of this fermentation was found to be 1.3-fold higher than a control fed-batch process. Characterization of the resin through isotherm analysis produced data that fit well (R-2 > 0.99) to both Langmuir and Redlich-Petersen models with a Langmuir monolayer loading of 203.8 mg acid/g resin. Resin stability was tested over 108 days of fermentation on corn stover hydrolysate. During this fermentation, resin capacities for lactic and acetic acid were on average 112.2 mg/g and 19.6 mg/g, respectively, with no statistical evidence for change lactic acid capacity after reuse. However, acetic acid capacity on average dropped 4.9% per reuse. (C) 2014 Published by Elsevier B.V.
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