Journal
BIOCHEMICAL ENGINEERING JOURNAL
Volume 95, Issue -, Pages 56-61Publisher
ELSEVIER
DOI: 10.1016/j.bej.2014.12.008
Keywords
Whole cell; Glucose; Biocatalysis; Transesterification; Optimisation
Funding
- National Natural Science Foundation of China [31270636, 20906031]
- Foundation for Natural Science Foundation of Guangdong Province [S2013010012486]
- Project of Department of Education of Guangdong Province [2012KJCX0006]
- Program for New Century Excellent Talents in University [NCET-12-0192]
- SCUT from the college basic research and operation of MOE [2013ZM0044, 2013ZZ0068, 2013ZM0065]
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For the first time, whole microbial-cell biocatalysis method was successfully applied by us to the production of glucose esters by transesterification of glucose with vinyl propionate. Among ten bacterial and fungal strains tested, lyophilized cells of Pseudomonas stutzeri strain GIM 1.273 efficiently catalyzed the reaction with the highest conversion and initial rate. (CNMR)-C-13 analysis showed that the reaction catalyzed by P. stutzeri yielded 6-O regiomer predominantly (>99%), clearly indicating high regioselectivity of the biocatalyst toward the 6-O-hydroxy group of glucose. Efficiency of the reaction catalyzed by lyophilized cells was evidently solvent-dependent. With the exception of acetonitrile-pyridine and tetrahydrofuran-pyridine systems, the substrate conversion rate clearly increased with increasing hydrophobicity of the organic solvent media used. The best results were obtained in isooctane-pyridine (3:7 v/v) system. Other optimal conditions were: water content 2% (v/v), molar ratio of the acyl donor to glucose 10:1, biocatalyst dosage 80 mg/mL and reaction temperature 35 degrees C, under which glucose conversion and reaction rate were 97.2% and 29.7 mmol/Lh over the duration of 24 h. A tenfold scale-up production of the 6-O-glucose ester further demonstrated that whole-cell P. stutzeri was an efficient and highly-regioselective alternative to purified enzymes for synthesis of glucose esters. (C) 2014 Elsevier B.V. All rights reserved.
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