Journal
MICROBIAL BIOTECHNOLOGY
Volume 7, Issue 2, Pages 90-99Publisher
WILEY
DOI: 10.1111/1751-7915.12092
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Funding
- Cultivation Foundation of Education Ministry, China [708034]
- Science and Technology Commission of Shanghai Municipality [13ZR1459000]
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Xylose fermentation is necessary for the bioconversion of lignocellulose to ethanol as fuel, but wild-type Saccharomyces cerevisiae strains cannot fully metabolize xylose. Several efforts have been made to obtain microbial strains with enhanced xylose fermentation. However, xylose fermentation remains a serious challenge because of the complexity of lignocellulosic biomass hydrolysates. Genome shuffling has been widely used for the rapid improvement of industrially important microbial strains. After two rounds of genome shuffling, a genetically stable, high-ethanol-producing strain was obtained. Designated as TJ2-3, this strain could ferment xylose and produce 1.5 times more ethanol than wild-type Pichiastipitis after fermentation for 96h. The acridine orange and propidium iodide uptake assays showed that the maintenance of yeast cell membrane integrity is important for ethanol fermentation. This study highlights the importance of genome shuffling in P.stipitis as an effective method for enhancing the productivity of industrial strains.
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