Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 465, Issue 1, Pages 88-94Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2015.07.135
Keywords
Circadian rhythm; N6-methyladenosine; Cryptochrome; RNA
Categories
Funding
- National Health Research Institute [NHRI-EX100-9925SC]
- National Science Council [101-2314-B-182A-009, 101-2314-B-182A-098-MY3, 103-2314-B-182A-092-MY3]
- Chang Gung Memorial Hospital [CMPRG3B1643, CMRPG3C1762, CMRPG3D0581, CMRPG3D1002, CMRPG3C0722]
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Methylation of RNA N-6-methyladenosine has fundamental cellular functions, including translation regulation, RNA export, and stem cells renewal. However, the regulation of RNA N-6-methyladenosine methylation is poorly understood. Here, We observed a robust circadian rhythm in N-6-methyladenosine modifications of RNA. Deficiency of core mammalian clock genes, cryptochromes, decreased the levels of N-6-methyladenosine in RNA. Cryptochrome1/2 knockout mice had significantly lower N-6-methyladenosine methylation of RNA and lost the circadian rhythm of N-6-methyladenosine levels in RNA. Global analysis of the circadian methylomes of N-6-methyladenosine in RNA revealed that gene transcription, translation regulation, and RNA metabolism were highly correlated with N-6-methyladenosine oscillation. Our findings extended a fundamental link between the circadian rhythm and N-6-methyladenosine modification of RNA and suggested that this link is critical in controlling post-transcriptional gene expression and RNA metabolism. (C) 2015 Elsevier Inc. All rights reserved.
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