Journal
CANCERS
Volume 10, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/cancers10080254
Keywords
TGF-beta RII receptor; STAT3; metastasis; gemcitabine; ABC transporters; pancreas
Categories
Funding
- Lille University/Faculty of Medicine
- Centre Hospitalier Regional et Universitaire (CHRU) de Lille/region Nord-Pas de Calais
- University of Lille 2
- ANR DRUG_MUC4
- Contrat Hospitalier de Recherche Translationnelle/CHRT, AVIESAN
- Inserm
- CNRS
- la Ligue Nationale Contre le Cancer (Equipe Labellisee Ligue)
- la Ligue Nationale Contre le Cancer (Ligue comite 59)
- la Ligue Nationale Contre le Cancer (Ligue comite 62, 80)
- SIRIC ONCOLille [INCaDGOS-Inserm 6041]
- Contrat de Plan Etat Region CPER Cancer
Ask authors/readers for more resources
Pancreatic adenocarcinoma (PDAC) is one of the most deadly cancers in the Western world because of a lack of early diagnostic markers and efficient therapeutics. At the time of diagnosis, more than 80% of patients have metastasis or locally advanced cancer and are therefore not eligible for surgical resection. Pancreatic cancer cells also harbour a high resistance to chemotherapeutic drugs such as gemcitabine that is one of the main palliative treatments for PDAC. Proteins involved in TGF-beta signaling pathway (SMAD4 or TGF-beta RII) are frequently mutated in PDAC (50-80%). TGF-beta signalling pathway plays antagonistic roles during carcinogenesis by initially inhibiting epithelial growth and later promoting the progression of advanced tumors and thus emerged as both tumor suppressor and oncogenic pathways. In order to decipher the role of TGF-beta in pancreatic carcinogenesis and chemoresistance, we generated CAPAN-1 and CAPAN-2 cell lines knocked down for TGF-beta RII (first actor of TGF-beta signaling). The impact on biological properties of these TGF-beta RII-KD cells was studied both in vitro and in vivo. We show that TGF-beta RII silencing alters tumor growth and migration as well as resistance to gemcitabine. TGF-beta RII silencing also leads to S727 STAT3 and S63 c-Jun phosphorylation, decrease of MRP3 and increase of MRP4 ABC transporter expression and induction of a partial EMT phenotype. These markers associated with TGF-beta signaling pathways may thus appear as potent therapeutic tools to better treat/manage pancreatic cancer.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available