Journal
ACTA PHARMACEUTICA SINICA B
Volume 1, Issue 4, Pages 248-253Publisher
INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
DOI: 10.1016/j.apsb.2011.10.005
Keywords
Gemifloxacin mesylate; Spectrophotometry; 1,2-Naphthoquinone-4-sulphonate; Pharmaceutical formulations
Categories
Ask authors/readers for more resources
This paper describes two simple spectrophotometric methods for the determination of the antibiotic gemifloxacin mesylate (GFX) in pharmaceutical formulations. The first (A) is an indirect method in which oxidation of the drug with a known excess of cerium (IV) sulphate is followed by determination of the residual oxidant by adding excess methyl orange and measuring residual dye at 507 nm. The second (B) is a derivatisation method involving reaction of GFX with 1,2-naphthoquinone-4-sulphonate (NQS) in alkaline medium (pH 11) to form an orange-coloured product exhibiting maximum absorption (lambda(max)) at 411 nm. The methods were linear in the concentration ranges 2-9 and 5-30 mu g/mL for methods A and B, respectively, with intra-day precision (as RSD) <1.5 A for both. When applied to the determination of GFX in pharmaceutical tablets, the results were in good agreement with those obtained by capillary electrophoresis. The two methods are useful for routine analysis of GFX in quality control laboratories. (C) 2011 Institute of Materia Medica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association. Production and hosting by Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available