Journal
SCIENCE ADVANCES
Volume 4, Issue 8, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aat5107
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Funding
- Army Research Office [W911NF-16-1-0372]
- NSF [MCB-1413563, MCB-1716766]
- Air Force Research Laboratory Center of Excellence [FA8650-15-2-5518]
- Defense Threat Reduction Agency [HDTRA1-15-10052/P00001]
- David and Lucile Packard Foundation
- Camille Dreyfus Teacher-Scholar Program
- U.S. Department of Energy BER (Biological and Environmental Research) grant [DE-SC0018249]
- Wyss Institute
- Paul G. Allen Frontiers Group
- Air Force Office of Scientific Research
- Natural Sciences and Engineering Council of Canada [RGPIN-2016-06352]
- NSF Graduate Research Fellowship
- Wyss Technology Development Fellowship
- Northwestern University Chemistry of Life Processes Summer Scholars program
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Synthetic biology offers opportunities for experiential educational activities at the intersection of the life sciences, engineering, and design. However, implementation of hands-on biology activities in classrooms is challenging because of the need for specialized equipment and expertise to grow living cells. We present BioBits (TM) Bright, a shelf stable, just-add-water synthetic biology education kit with easy visual outputs enabled by expression of fluorescent proteins in freeze-dried, cell-free reactions. We introduce activities and supporting curricula for teaching the central dogma, tunable protein expression, and design-build-test cycles and report data generated by K-12 teachers and students. We also develop inexpensive incubators and imagers, resulting in a comprehensive kit costing
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