Journal
ACS SENSORS
Volume 3, Issue 10, Pages 2138-2144Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b00701
Keywords
hydrogen sulfide; Hemoglobin I; fluorescent sensor; absorption into fluorescence; Lucina pectinata; biosensor
Ask authors/readers for more resources
A new detection system for the endogenous gaseous transmitter and environmental pollutant hydrogen sulfide is presented. It is based on the modulation of the fluorescence spectrum of a coumarin dye by the absorption spectrum of the recombinant hemoglobin I from clam Lucina pectinata upon coordination of the analyte. While we establish that the reported affinity of rHbI for H2S has been overestimated, the association of the protein with an appropriate fluorophore allows fast, easy, and reversible detection and quantification of hydrogen sulfide in buffer as well as biological fluids such as human plasma, with a quantification limit around 200 nM at pH 7.4.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available