Journal
CHINESE SCIENCE BULLETIN
Volume 54, Issue 7, Pages 1210-1216Publisher
SCIENCE PRESS
DOI: 10.1007/s11434-009-0142-2
Keywords
Upland cotton; fibroin; Agrobacterium-mediated transformation; surface microstructure of cotton fiber; fiber quality
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Funding
- National High Technology Research and Development Program of China [2006AA100105]
- Science & Technology Pillar Program of Jiangsu Province [BE2008310]
- Programme of Introducing Talents of Discipline to Universities [B08025)]
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A silkworm gene for fibroin was introduced into the upland cotton WC line by Agrobacterium-mediated transformation. PCR detection for fibroin, nptII and gus genes, Kanamycin (Km)-resistance analysis and GUS-histochemical assay were conducted on 30 regenerated plants from 9 callus lines, and 17 positive plants were obtained by these 5 screening methods. By Km-resistance analysis and PCR for fibroin, 6 homozygous lines in T-3 were obtained. Southern blot and Northern bolt demonstrated that the fibroin gene was inserted into the genome of these 6 lines, stably inherited and expressed. Compared to the control, the surface structure of mature fiber in the 6 lines was significantly distorted and an increased number of convolution was observed by scanning electron microscopy (SEM). Fiber quality traits analysis indicated that fiber elongation of the 6 homozygous lines was all increased and fiber strength of 3 lines was enhanced. These results indicated that fibroin expression influenced cotton fiber structure and quality, suggesting that fibroin has great potential for improving cotton fiber quality by genetic engineering.
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