Journal
REDOX BIOLOGY
Volume 2, Issue -, Pages 619-631Publisher
ELSEVIER
DOI: 10.1016/j.redox.2014.03.006
Keywords
Cybrid; Mitochondria; Mitochondrial DNA; Rho zero
Categories
Funding
- University of Kansas Alzheimer's Disease Center [P30AG035982]
- Frank and Evangeline Thompson Alzheimer's Treatment Program Fund
- NATIONAL INSTITUTE ON AGING [P30AG035982] Funding Source: NIH RePORTER
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Cytoplasmic hybrid (cybrid) cell lines can incorporate human subject mitochondria and perpetuate its mitochondrial DNA (mtDNA)-encoded components. Since the nuclear background of different cybrid lines can be kept constant, this technique allows investigators to study the influence of mtDNA on cell function. Prior use of cybrids has elucidated the contribution of mtDNA to a variety of biochemical parameters, including electron transport chain activities, bioenergetic fluxes, and free radical production. While the interpretation of data generated from cybrid cell lines has technical limitations, cybrids have contributed valuable insight into the relationship between mtDNA and phenotype alterations. This review discusses the creation of the cybrid technique and subsequent data obtained from cybrid applications. (C) 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
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