Journal
PROBIOTICS AND ANTIMICROBIAL PROTEINS
Volume 1, Issue 2, Pages 121-129Publisher
SPRINGER
DOI: 10.1007/s12602-009-9015-x
Keywords
Bifidobacteria; Prebiotics; Arabinoxylan; Endoxylanase gene; Real-time reverse transcription-PCR; Quantitative analysis
Categories
Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC) grant
- Government of Canada (via Canada Research Chair in Lactic Culture Biotechnology for Dairy and Probiotic Industries)
- NSERC fellowship
Ask authors/readers for more resources
Real-time quantitative PCR (qRT-PCR) can be used to monitor specific catabolic activity by gene transcriptional analysis of bacterial cultures. This methodology has been applied to determine if the differential expression of genes putatively involved in arabinoxylan degradation by Bifidobacteriumlongum NCC2705 could be associated to the consumption of this prebiotic. Three genes putatively encoding arabinofuranosidases (abfI, abfA, and abfB) and one putatively encoding endoxylanase (xynD) were targeted for this purpose. Bifidobacteriumlongum NCC2705 exhibited higher growth yield relative to glucose based on viable counts or optical density for arabinoxylan as compared to xylose and arabinose. Among reference genes studied (16S rRNA, tufA, recA, rpoB, and atpD) the most stably expressed genes were rpoB, tufA, and atpD. The most significant increase in target gene expression was observed in the presence of arabinoxylan for the xynD gene, while xylose and arabinose had a weaker effect on xynD expression. In conclusion, B. longum NCC2705 over expresses an endoxylanase gene in response to arabinoxylan.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available