Journal
CENTRAL EUROPEAN JOURNAL OF BIOLOGY
Volume 8, Issue 3, Pages 259-262Publisher
VERSITA
DOI: 10.2478/s11535-013-0136-y
Keywords
Candida albicans; Cell surface hydrophobicity; Biofilm; Planktonic cells; Dispersal cells
Categories
Funding
- Slovak Ministry of Education [VEGA 1/0966/12]
- Slovak Research and Development Agency [APVV-0291-11]
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Overall cell surface hydrophobicity (CSH) is predicted to play an important role during biofilm formation in Candida albicans but is the result of many expressed proteins. This study compares the CSH status and CSH1 gene expression in C. albicans planktonic cells, sessile biofilm, and dispersal cells. Greater percentages of hydrophobic cells were found in non-adhered (1.5 h) and dispersal forms (24 or 48 h) (41.34 +/- 4.17% and 39.52 +/- 7.45%, respectively), compared with overnight planktonic cultures (21.69 +/- 3.60%). Results from quantitative real-time PCR confirmed greater up-regulation of the CSH1 gene in sessile biofilm compared with both planktonic culture and dispersal cells. Up-regulation was also greater in dispersal cells compared with planktonic culture. The markedly increased CSH found both in C. albicans biofilm, and in cells released during biofilm formation could provide an advantage to dispersing cells building new biofilm.
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