Journal
CENTRAL EUROPEAN JOURNAL OF CHEMISTRY
Volume 8, Issue 1, Pages 87-95Publisher
SCIENDO
DOI: 10.2478/s11532-009-0125-9
Keywords
Pressurised Fluid Extraction; Ultrasonic Extraction; Antioxidants; Antioxidant Capacity; High-Performance Liquid Chromatography
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Funding
- Ministry of Education, Youth and Sports of the Czech Republic [MSM 0021627502]
- Czech Science Foundation [203/08/1536]
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The analytical method based on the high-performance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin, 5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL(-1) (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43% of DPPH radical inhibition for PFE method and from 76.01 to 89.94% in the case of USE method.
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