Journal
ONCOIMMUNOLOGY
Volume 3, Issue 12, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/21624011.2014.968467
Keywords
cancer peptide vaccines; complementary determining region 3; immune responses; next-generation sequencing; T cell repertoire
Categories
Funding
- National Institutes of Health in Clinical Therapeutics [T32 GM007019]
Ask authors/readers for more resources
Immune responses play a critical role in various disease conditions including cancer and autoimmune diseases. However, to date, there has not been a rapid, sensitive, comprehensive, and quantitative analysis method to examine T-cell or B-cell immune responses. Here, we report a new approach to characterize T cell receptor (TCR) repertoire by sequencing millions of cDNA of TCR alpha and beta chains in combination with a newly-developed algorithm. Using samples from lung cancer patients treated with cancer peptide vaccines as a model, we demonstrate that detailed information of the V-(D)-J combination along with complementary determining region 3 (CDR3) sequences can be determined. We identified extensive abnormal splicing of TCR transcripts in lung cancer samples, indicating the dysfunctional splicing machinery in T lymphocytes by prior chemotherapy. In addition, we found three potentially novel TCR exons that have not been described previously in the reference genome. This newly developed TCR NGS platform can be applied to better understand immune responses in many disease areas including immune disorders, allergies, and organ transplantations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available