Journal
NEOPLASIA
Volume 16, Issue 5, Pages 432-440Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.neo.2014.05.005
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Funding
- MSKCC Metastasis Research Center
- Radiochemistry and Molecular Imaging Probes Core at Memorial Sloan-Kettering Cancer Center [P30 CA008748]
- NIH [K25 EB016673]
- Brain Tumor Center of Memorial Sloan-Kettering Cancer Center
- Radiology Development Fund
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New intravital optical imaging technologies have revolutionized our understanding of mammalian biology and continue to evolve rapidly. However, there are only a limited number of imaging probes available to date. In this study, we investigated in mouse models of glioblastoma whether a fluorescent small molecule inhibitor of the DNA repair enzyme PARP1, PARPi-FL, can be used as an imaging agent to detect glioblastomas in vivo. We demonstrated that PARPi-FL has appropriate biophysical properties, low toxicity at concentrations used for imaging, high stability in vivo, and accumulates selectively in glioblastomas due to high PARP1 expression. Importantly, subcutaneous and orthotopic glioblastoma xenografts were imaged with high contrast clearly defining tumor tissue from normal surrounding tissue. This research represents a step toward exploring and developing PARPi-FL as an optical intraoperative imaging agent for PARP1 in the clinic.
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