Journal
NEOPLASIA
Volume 14, Issue 11, Pages 1067-U206Publisher
ELSEVIER SCIENCE INC
DOI: 10.1593/neo.121322
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Funding
- National Institutes of Health [R01 CA107469, R01 CA125577, U01CA154224]
- University of Michigan's Cancer Center [5 P30 CA46592]
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While normal cells in the human breast are organized into acinar structures, disruption of the acinar architecture is a hallmark of cancer. In a three-dimensional model of morphogenesis, we show that down-regulation of the matrix-associated tumor suppressor protein CCN6 (WNT1-inducible-signaling pathway protein 3) disrupts breast epithelial cell polarity and organization into acini through up-regulation of the type III transforming growth factor-beta receptor (T beta RIII or betaglycan). Down-regulation of CCN6 in benign breast cells led to loss of tissue polarity and resulted in cellular disorganization with loss of alpha 6 integrin-rich basement membrane and the basolateral polarity protein E-cadherin. Silencing of T beta RIII with shRNA and siRNA rescued the ability of breast epithelial cells to form polarized acinar structures with reduced matrix invasion and restored the correct expression of alpha 6 integrin and E-cadherin. Conversely, CCN6 overexpression in aggressive breast cancer cells reduced T beta RIII in vitro and in a xenograft model of CCN6 overexpression. The relevance of our studies to human breast cancer is highlighted by the finding that CCN6 protein levels are inversely associated with T beta RIII protein in 64% of invasive breast carcinomas. These results reveal a novel function of the matricellular protein CCN6 and establish a mechanistic link between CCN6 and T beta RIII in maintaining acinar organization in the breast. Neoplasia (2012) 14, 1067-1074
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