Journal
STRUCTURAL DYNAMICS-US
Volume 2, Issue 4, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4929410
Keywords
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Funding
- U.S. National Science Foundation (NSF) [1120997]
- NSF STC BioXFEL center Award [1231306]
- National Institutes of Health (NIH) [GM097463-04, GM108635, 1R01GM095583]
- PSI:Biology Center MPID [U54GM094625]
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Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with similar to 700 angstrom diameter. Microcrystals delivered in viscous agarose medium diffracted to similar to 40 angstrom resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is an important step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses. (C) 2015 Author(s).
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