Journal
MYCOBIOLOGY
Volume 42, Issue 3, Pages 256-261Publisher
TAYLOR & FRANCIS LTD
DOI: 10.5941/MYCO.2014.42.3.256
Keywords
Bioconversion; Compound K; Red ginseng extract; Saccharomyces cerevisiae HJ-014
Funding
- RIC program of MOTIE (Ministry of Trade, Industry and Energy) in Daejeon University
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A beta-glucosidase producing yeast strain was isolated from Korean traditional rice wine. Based on the sequence of the YCL008c gene and analysis of the fatty acid composition, the isolate was identified as Saccharomyces cerevisiae strain HJ-014. S. cerevisiae HJ-014 produced ginsenoside Rd, F-2, and compound K from the ethanol extract of red ginseng. The production was increased by shaking culture, where the bioconversion efficiency was increased 2-fold compared to standing culture. The production of ginsenoside F-2 and compound K was time-dependent and thought to proceed by the transformation pathway of: red ginseng extract -> Rd -> F-2 -> compound K. The optimum incubation time and concentration of red ginseng extract for the production of compound K was 96 hr and 4.5% (w/v), respectively.
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