Journal
MOLECULAR METABOLISM
Volume 3, Issue 4, Pages 490-494Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molmet.2014.04.007
Keywords
Brown adipose tissue; Brown fat; BAT; Uncoupling protein-1; Obesity; Diabetes; Metabolism; Thermography; Beige fat; Brite fat
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The activation and expansion of brown adipose tissue (BAT) has emerged as a promising strategy to counter obesity and the metabolic syndrome by increasing energy expenditure. The subsequent testing and validation of novel agents that augment BAT necessitates accurate pre-clinical measurements in rodents regarding the capacity for BAT-derived thermogenesis. We present a novel method to measure BAT thermogenesis using infrared imaging following beta(3)-adrenoreceptor stimulation in mice. We show that the increased body surface temperature observed using this method is due solely to uncoupling protein-1 (UCP1)-mediated thermogenesis and that this technique is able to discern differences in BAT activity in mice acclimated to 23 degrees C or thermoneutrality (30 degrees C). These findings represent the first standardized method utilizing infrared imaging to specifically detect UCP1 activity in vivo. (C) 2014 The Authors. Published by Elsevier GmbH.
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