Journal
METHODS AND APPLICATIONS IN FLUORESCENCE
Volume 6, Issue 4, Pages -Publisher
IOP Publishing Ltd
DOI: 10.1088/2050-6120/aadef1
Keywords
lanthanides; photophysics; upconversion; luminescence modulation; imaging
Categories
Funding
- 'Center for Synthetic Biology' at Copenhagen University - UNIK research initiative of the Danish Ministry of Science, Technology and Innovation [09-065274]
- bioSYNergy, University of Copenhagen's Excellence Programme for Interdisciplinary Research
- Villum Foundation [VKR023115]
- Carlsberg Foundation [CF14-0388]
- Danish Council of Independent Research [DFF-7014-00027]
- Villum Fonden [00007264] Funding Source: researchfish
Ask authors/readers for more resources
Upon direct excitation with green light (522 nm), Er3+ ion doped nanoparticles feature a number of radiative and non-radiative decay pathways, leading to distinct and sharp emission lines in the visible and near-infrared (NIR) range. Here we apply, in addition to continuous 522 nm irradiation, a modulated NIR irradiation (1143 nm) to actively control and modulate the red emission intensity (around 650 nm). The modulation of red Er3+ ion emission at a chosen frequency allows us to reconstruct fluorescence images from the Fourier transform amplitude at this particular frequency. Since only the emission from the Er3+ ion is modulated, it allows to selectively recover the lanthanide specific signal, removing any non-modulated auto-fluorescence or background emission resulting from the continuous 522 nm excitation. The modulated emission of specific lanthanides can open up new detection opportunities for selective signal recovery.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available