Journal
JOURNAL OF SYNCHROTRON RADIATION
Volume 21, Issue -, Pages 24-31Publisher
WILEY-BLACKWELL
DOI: 10.1107/S1600577513024715
Keywords
structural biology; footprinting; X-rays; beamlines; radiolysis
Categories
Funding
- National Institute for Biomedical Imaging and Bio-engineering [P30-EB-09998, R01-EB-09688]
- National Science Foundation [DBI-1228549]
- US Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-98CH10886]
- Office of Science, Office of Basic Energy Sciences, of the US Department of Energy [DE-AC02-05CH11231]
- US DOE [DE-AC02-06CH11357]
- National Institutes of Health/National Institute of General Medical Sciences under NSF [DMR-0936384]
- Direct For Biological Sciences
- Div Of Biological Infrastructure [1228549] Funding Source: National Science Foundation
Ask authors/readers for more resources
Synchrotron footprinting is a valuable technique in structural biology for understanding macromolecular solution-state structure and dynamics of proteins and nucleic acids. Although an extremely powerful tool, there is currently only a single facility in the USA, the X28C beamline at the National Synchrotron Light Source (NSLS), dedicated to providing infrastructure, technology development and support for these studies. The high flux density of the focused white beam and variety of specialized exposure environments available at X28C enables footprinting of highly complex biological systems; however, it is likely that a significant fraction of interesting experiments could be performed at unspecialized facilities. In an effort to investigate the viability of a beamline-flexible footprinting program, a standard sample was taken on tour around the nation to be exposed at several US synchrotrons. This work describes how a relatively simple and transportable apparatus can allow beamlines at the NSLS, CHESS, APS and ALS to be used for synchrotron footprinting in a general user mode that can provide useful results.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available