Journal
SCIENCE BULLETIN
Volume 60, Issue 4, Pages 419-427Publisher
ELSEVIER
DOI: 10.1007/s11434-014-0659-x
Keywords
Hydrogen sulfide (H2S); K+ current; Extracellular ATP (eATP); Hydrogen peroxide (H2O2); Stomatal closure; Arabidopsis thaliana
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Funding
- National Natural Science Foundation of China [31170237]
- National Key Laboratory Program of China Agricultural University [SKLPPBKF11001]
- Shandong Taishan Scholar Program
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Hydrogen sulfide (H2S) is a newly discovered gaseous signaling molecule and involved in ethylene and ABA-induced stomatal closure. As an important factor, extracellular ATP (eATP) was believed to participate in regulation of stomatal closing. However, the mechanism by which eATP mediates H2S-regulated stomatal closure remains unclear. Here, we employed Arabidopsis wild-type and mutant lines of ATP-binding cassette transporters (Atmrp4, Atmrp5 and their double mutant Atmrp4/5) to study the function of eATP in H2S-regulated stomatal movement. Our results indicated that H2S affected stomatal closing through stimulating guard cell outward K+ current. Moreover, we found that H2S induced eATP generation by regulating the activity of an ABC transporter. The inhibitor of ABC transporters, glibenclamide (Gli), could impair H2S-regulated stomatal closure and reduce H2S-dependent eATP accumulation in Atmrp4 and Atmrp5 mutants. In addition, the promotion effect of H2S on outward K+ currents was diminished in Atmrp4/5 double mutant. Our data suggested that hydrogen peroxide (H2O2) is required for H2S-induced stomatal closure, and the production of H2O2 is regulated by eATP via NADPH oxidase. Based on this work, we conclude that H2S-induced stomatal closure requires ABC transporter-dependent eATP production and subsequent NADPH oxidase-dependent H2O2 accumulation.
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