Journal
BIOENGINEERED
Volume 4, Issue 1, Pages 9-14Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/bioe.21950
Keywords
influenza virus; reverse genetics; viral vector; gene delivery; pseudo-typed virus; microRNA delivery; vaccine; gene therapy
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Funding
- National Institute of Allergy and Infectious Diseases, NIH [R01AI072139]
- Texas Tech University Health Sciences Center Paul L. Foster School of Medicine
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The influenza virus is a respiratory pathogen with a negative-sense, segmented RNA genome. Construction of recombinant influenza viruses in the laboratory was reported starting in the 1980s. Within a short period of time, pioneer researchers had devised methods that made it possible to construct influenza viral vectors from cDNA plasmid systems. Herein, we discuss the evolution of influenza virus reverse genetics, from helper virus-dependent systems, to helper virus-independent 17-plasmid systems, and all the way to 3- and 1-plasmid systems. Successes in the modification of different gene segments for various applications, including vaccine and gene therapies are highlighted.
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