Journal
BIOENGINEERED
Volume 4, Issue 6, Pages 401-407Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/bioe.24094
Keywords
Listeria; osmotolerance; chill-tolerance; salt stress; twisted cells
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Funding
- IRCSET EMBARK Postgraduate Scholarship [RS/2010/2300]
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Betaine uptake in Listeria monocytogenes is mediated by three independent transport systems, the simplest of which in genetic terms is the secondary transporter BetL. Using a random mutagenesis approach, based on the E. coli XL1 Red mutator strain, we identified a single point mutation in a putative promoter region upstream of the BetL coding region which leads to a significant increase in betL transcript levels under osmo- and chill-stress conditions and a concomitant increase in stress tolerance. Furthermore, the mutation appears to counter the heretofore unreported twisted cell morphology observed for L. monocytogenes grown at elevated osmolarities in tryptone soy broth.
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