Journal
AMB EXPRESS
Volume 4, Issue -, Pages -Publisher
SPRINGEROPEN
DOI: 10.1186/s13568-014-0020-4
Keywords
Corynebacterium glutamicum; Gamma-aminobutyric acid; Glutamate decarboxylase; 2-oxoglutarate dehydrogenase; Protein kinase G
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
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Gamma-aminobutyric acid (GABA), a building block of the biodegradable plastic polyamide 4, is synthesized from glucose by Corynebacterium glutamicum that expresses Escherichia coli glutamate decarboxylase (GAD) B encoded by gadB. This strain was engineered to produce GABA more efficiently from biomass-derived sugars. To enhance GABA production further by increasing the intracellular concentration of its precursor glutamate, we focused on engineering pknG (encoding serine/threonine protein kinase G), which controls the activity of 2-oxoglutarate dehydrogenase (Odh) in the tricarboxylic acid cycle branch point leading to glutamate synthesis. We succeeded in expressing GadB in a C. glutamicum strain harboring a deletion of pknG. C. glutamicum strains GAD and GAD Delta pknG were cultured in GP2 medium containing 100 g L-1 glucose and 0.1 mM pyridoxal 5'-phosphate. Strain GAD Delta pknG produced 31.1 +/- 0.41 g L-1 (0.259 g L-1 h(-1)) of GABA in 120 hours, representing a 2.29-fold higher level compared with GAD. The production yield of GABA from glucose by GAD Delta pknG reached 0.893 mol mol(-1).
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