Journal
3 BIOTECH
Volume 5, Issue 2, Pages 221-225Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-014-0212-2
Keywords
Accessory enzyme; Fungi; Hemicellulase; beta-Mannanase; Saccharification; Softwood
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Funding
- Scion core funding in Bioenergy
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Commercially available enzymes do not contain all the necessary softwood-specific accessory enzymes to obtain high saccharification efficiency. In this work, six saprophytic fungi obtained from Pinus radiata plantation site were screened for the putative softwood-specific accessory enzyme, beta-mannanase. A Penicillium sp. was found to produce beta-mannanase in both solid (31.6 units/g of dry biomass) and liquid (117 units/g of dry biomass) cultures using locust bean gum as an inducer after 2 weeks of incubation. The saccharification of steam-exploded Pinus radiata was 7.8 % w/w improved when the crude extract of Penicillium sp. was added to a mixture of commercial enzymes.
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