Bioethanol Production from the Raw Corn Starch and Food Waste Employing Simultaneous Saccharification and Fermentation Approach

Response surface methodology based on the 2(4) factorial central composite design (CCD) was applied to optimize the liquefaction and saccharification process for the enhancement of sugar production from raw corn starch. Optimum level of the pre and post-cooking alpha-amylase and glucoamylase dose were found to be 3.0 and 1.5 U/ml, respectively, while saccharification temperature for the maximum sugar production was found to be 50 A degrees C. The quadratic model predicted that maximum conversion efficiency for the enhanced sugar production from raw corn starch was 93.65 % under the above mentioned optimum level of variables. Additional experiments performed in triplicate with the optimized level of variables showed maximum conversion efficiency of 94.12 %, in close agreement between the predicted and experimental results. The generated sugar was subjected for the bioethanol production in separate fermentation system using yeast while simultaneous saccharification and fermentation system was also evaluated.