Journal
ONCOIMMUNOLOGY
Volume 4, Issue 6, Pages -Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/2162402X.2015.1008805
Keywords
immune escape; microRNA; non-classical HLA class I molecules; renal cell carcinoma; tumor-infiltrating lymphocytes
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Funding
- Deutsche Forschungsgemeinschaft [DFG 585-9-2, 11-2]
- DFG GRK [1591]
- German Israelian Foundation [I-1187-69.11/2012]
- German Cancer Aid [111105, 107967]
- intramural Roux program of the Martin Luther University Halle-Wittenberg
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In human tumors of distinct origin including renal cell carcinoma (RCC), the non-classical human leukocyte antigen G (HLA-G) is frequently expressed, thereby inhibiting the cytotoxic activity of T and natural killer (NK) cells. Recent studies demonstrated a strong post-transcriptional gene regulation of the HLA-G by miR-152, -148A, -148B and -133A. Standard methods were applied to characterize the expression and function of HLA-G, HLA-G-regulatory microRNAs (miRs) and the immune cell infiltration in 453 RCC lesions using a tissue microarray and five RCC cell lines linking these results to clinical parameters. Direct interactions with HLA-G regulatory miRs and the HLA-G 3 untranslated region (UTR) were detected and the affinities of these different miRs to the HLA-G 3-UTR compared. qPCR analyses and immunohistochemical staining revealed an inverse expression of miR-148A and -133A with the HLA-G protein in situ and in vitro. Stable miR overexpression caused a downregulation of HLA-G protein enhancing the NK and LAK cell-mediated cytotoxicity in in vitro CD107a activation assays revealing a HLA-G-dependent cytotoxic activity of immune effector cells. A significant higher frequency of CD3(+)/CD8(+) T cell lymphocytes, but no differences in the activation markers CD69, CD25 or in the presence of CD56(+), FoxP3(+) and CD4(+) immune cells were detected in HLA-G(+) compared to HLA-G(-) RCC lesions. This could be associated with higher WHO grade, but not with a disease-specific survival. These data suggest a miR-mediated control of HLA-G expression in RCC, which is associated with a distinct pattern of immune cell infiltration.
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