Journal
MOLECULAR THERAPY-NUCLEIC ACIDS
Volume 13, Issue -, Pages 312-321Publisher
CELL PRESS
DOI: 10.1016/j.omtn.2018.09.010
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Funding
- NIH [CA190040, CA211457, DK118250]
- Emerson Research Foundation
- Key Science and Technology Program of Shaanxi Province, China [2015SF128]
- International Scientific and Technological Cooperation and Exchange Program of Shaanxi Province, China [2015KW-030]
- Hopkins Digestive Diseases Basic and Translational Research Core Center (NIDDK center) [P30 DK089502]
- NATIONAL CANCER INSTITUTE [R01CA190040, UG3CA211457] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [P30DK089502, R01DK118250] Funding Source: NIH RePORTER
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MicroRNA (miR) sponges containing miR binding sequences constitute a potentially powerful molecular therapeutic strategy. Recently, naturally occurring circular RNAs (circRNAs) were shown to function as efficient miR sponges in cancer cells. We hypothesized that synthetic circRNA sponges could achieve therapeutic loss-of-function targeted against specific miRs. Linear RNA molecules containing miR-21 binding sites were transcribed in vitro; after dephosphorylation and phosphorylation, circularization was achieved using 5'-3' end-ligation by T4 RNA ligase 1. circRNA stability was assessed using RNase R and fetal bovine serum. Competitive inhibition of miR-21 activity by a synthetic circRNA sponge was assessed using luciferase reporter, cell proliferation, and cell apoptosis assays in three gastric cancer cell lines. circRNA effects on downstream proteins were also delineated by Tandem Mass Tag (TMT) labeling (data available via ProteomeXchange identifier PRIDE: PXD008584), followed by western blotting. We conclude that artificial circRNA sponges resistant to nuclease digestion can be synthesized using simple enzymatic ligation steps. These sponges inhibit cancer cell proliferation and suppress the activity of miR-21 on downstream protein targets, including the cancer protein DAXX. In summary, synthetic circRNA sponges represent a simple, effective, convenient strategy for achieving targeted loss of miR function in vitro, with potential future therapeutic application in human patients.
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