Journal
METHODS AND APPLICATIONS IN FLUORESCENCE
Volume 3, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2050-6120/3/2/024001
Keywords
single particle tracking; nanobody; single molecule; mHoneydew
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Funding
- Swiss National Fund
- European Commission Marie Curie Action [FP7-PEOPLE-2013-IEF 630024]
- School of Biomedical Sciences of King's College London
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Single particle tracking is a powerful tool to investigate the function of biological molecules by following their motion in space. However, the simultaneous tracking of two different species of molecules is still difficult to realize without compromising the length or density of trajectories, the localization accuracy or the simplicity of the assay. Here, we demonstrate a simple dual color single particle tracking assay using small, bright, high-affinity labeling via nanobodies of accessible targets with widely available instrumentation. We furthermore apply a ratiometric step-size analysis method to visualize differences in apparent membrane viscosity.
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